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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which leads to chromosome instability, and so may well add to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two more splice variants had been claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide typically contains the epitope acknowledged via the antibody.
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This information outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining with the blocked antibody as opposed to the antibody alone, one can see which staining is particular.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which lead towards the temporal coordination of each mitotic event. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S phase boundary which is degraded as cells progress via S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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